A large part of the collections today exhibited in the permanent exhibition or kept in the Museum’s storage area came from donors and sponsors who financed the purchase of artifacts. Already since the era that the Museum formed part of the 9th Ephorate of Byzantine Antiquities, before its becoming an independent Special Regional Service in 1997, but even before its foundation, the Museum has received several donations.
The first donation was realized in 1958 (completed in 1977), while the second one was in 1981. From the 1980s and on there is a constant flow of donations, which enrich the museum collection, almost on an annual basis, and demonstrate the active participation of citizens, of the local community and beyond, as well as their confidence in the work of the Museum Byzantine Culture. The bequests of two large private collections by Demetrios Economopoulos and Dori Papastratou stand out among them; part of these collections are presented in two separate rooms, which opened to the public on May 27th 2001. These special displays function as independent entities, while supplementing the museum's main thematic display, and they also allow visitors to perceive, through the choice of archaeological material, the collectors' sources, motives, and orientations when putting their collections together.
The museum's collections are also augmented by new acquisitions, mainly made with donations from private individuals and institutions, such as the J. F. Costopoulos Foundation.
Whether displayed in the permanent exhibition or kept in the Museum’s storage area awaiting their turn to be presented to the wide public, the artifacts that are until today donated to the Museum of Byzantine Culture surpass the 2000 (475 icons, 233 engravings and lithographs, 10 engraving matrixes, 42 works by modern and contemporary artists, 481 ceramic works, 687 coins, 75 metal objects, four fabrics and two early printed books), while the number of donors reaches the 40.
Recognizing the contribution of the donors and donors to the enrichment of the museum collection, the Museum organizes temporary exhibitions of small scale, displaying new its acquisitions (1999, 2002, 2007, 2014 retrospectively from 1977 to today, celebrating twenty year anniversary of the Museum).
The permanent display titled “The Twilight of Byzantium” was organized and its archaeological material conserved thanks to a generous donation by the Marinopoulos family and Carrefour-Marinopoulos SA in memory of Ioannis P. Marinopoulos.
Sponsors have also played a key part in the development of certain sections of the museum, such as the archaeological storerooms and the educational programs. The storerooms were organized in part with a donation from Papastratos SIA; while the educational programs were equipped and provided with pottery and mosaic workshops thanks to a donation from the A. G. Leventis Foundation, which also financed the production of printed material used by the instructors.
Moreover, the museum relies on sponsorships for the organization of events. The role of the Association of Friends of the Museum is crucial. Private companies have also contributed in this part, such as Cardico, Goodys Haitoglou Brothers SA, Theios Vanias SA, Cibus SA, Athinaiki Zythopoiia SA, MEVGAL, Passias SA, Pastry Shops Hatzis, Wine Roads, E. Tsantalis SA. Souroti Bottling and Trading Mineral Water Company SA, Polikos, Kempinski Hotel Nikopolis Thessaloniki etc.